Antibody Engineering: Advances in Phage Display Technology and in the Production of Therapeutic Immunocytokines
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منابع مشابه
Construction of Human Recombinant ScFv Phage Libraries from the Advanced Stages of Breast Carcinoma Patients
Advances in the field of antibody engineering, and the emergence of powerful screening technology such as filamentous phage display allowed to generate fully human antibodies with high affinities against virtually any desired target from immune or even naIve human repertoires. As a result, the immunogenicity problems related to applications of nonhuman based recombinant antibodies as therapeuti...
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Background and purpose: Antibody phage display library is a powerful in vitro technology for production of recombinant antibody fragments against a wide variety of antigens. However, the presence of insert-free clones in the phage libraries limited the specific enrichment of antibody fragments in many studies. The aim of this study was to protein A-aided recovery of insert-containing phages in ...
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Since the development of antibody-production techniques, a number of immunoglobulins have been developed on a large scale using conventional methods. Hybridoma technology opened a new horizon in the production of antibodies against target antigens of infectious pathogens, malignant diseases including autoimmune disorders, and numerous potent toxins. However, these clinical humanized or chimeric...
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Brain-Derived Neurotrophic Factor (BDNF) is a neuroprotectant candidate for neurodegenerative diseases. However, there are several clinical concerns about its therapeutic applications. In the current study, we selected BDNF-mimicking small peptides from phage-displayed peptide library as alternative molecules to the clinical challenges. The peptide library was screened against BDNF receptor (Ne...
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